海水浸泡對(duì)腹部開(kāi)放傷大鼠TGF-β_1、VEGF蛋白及mRNA表達(dá)的影響

摘要： 目的觀察海水浸泡對(duì)腹部開(kāi)放傷大鼠轉(zhuǎn)化生長(zhǎng)因子β_1(TGF-β_1)和血管內(nèi)皮生長(zhǎng)因子(VEGF)蛋白及mRNA表達(dá)的影響。方法成年健康Wistar大鼠60只,隨機(jī)分為2組,其中腹部開(kāi)放傷合并海水浸泡組40只,腹部開(kāi)放傷對(duì)照組20只。腹部開(kāi)放傷合并海水浸泡后0.5、1、2、3、4h應(yīng)用免疫組織化學(xué)、核酸原位雜交和圖像分析技術(shù)檢測(cè)大鼠肺和腎組織TGF-β_1和VEGF蛋白及mRNA表達(dá)水平的變化。結(jié)果對(duì)照組在致傷后1h開(kāi)始出現(xiàn)TGF-β_1蛋白陽(yáng)性表達(dá),2、3和4h表達(dá)逐漸增強(qiáng)。海水浸泡組0.5h開(kāi)始出現(xiàn)陽(yáng)性表達(dá),此后表達(dá)逐漸增強(qiáng),4h時(shí)陽(yáng)性表達(dá)量最高。TGF-β_1 mRNA表達(dá)滯后于蛋白表達(dá)。兩組均于致傷后2h開(kāi)始出現(xiàn)VEGF蛋白陽(yáng)性表達(dá),3、4h表達(dá)逐漸增強(qiáng)。VEGF mRNA與蛋白表達(dá)同步且一致。結(jié)論TGF-β_1和VEGF蛋白及mRNA的高表達(dá)與損傷關(guān)系密切,可作為海水浸泡傷預(yù)后判斷的指標(biāo)。
Objective To study the effects of seawater immersion on the expression of transforming growth factor-β_1(TGF-β_1)and vascular endothelial growth factor(VEGF)in lung and kidney tissues in open abdominal injury in rats.Methods Sixty adult Wistar rats were randomly divided into two groups,40 with open abdominal injury followed by seawater immersion group(seawater immersion group),20 with only open abdominal injury group(control group).The protein and mRNA expression of TGF-β_1 and VEGF of the lung and kidney tissues were examined by immunohistochemistry and situ hybridization in both groups at 0.5h,1 hour,2 hours,3 hours and 4 hours after the injury.Results In the control group,the TGF-β_1 protein began to express at 1h after injury,and the expression was grad- ually up-regulated at 2h,3h,4h.In seawater immersion group,the expression of TGF-β_1 protein significantly up-regulated at 0.5h,and the expression reached the peak value at 4 hours.The expression of TGF-β_1 mRNA lagged behind protein expression.In the control group,VEGF protein began to express at 2h after injury,and the expression was gradually up-regulated at 3h and 4h.In seawater immer- sion group,VEGF protein began to express at 2h after injury,and reached the peak value at 3h and 4h.VEGF mRNA and protein expres- sion was synchronous and consistent.Conclusions The expressions of TGF-β_1,VEGF mRNA and their proteins were closely related with type of injury,and they may serve as prognostic indices of seawater immersion injury.